Clone

Target

IDP00205  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

690  

Reference Sequence Used

MNKFVRLAAI AGLLLAGASY ATDTTYRIDQ LPQLRQEPEH ATVSERVTSR FTRSHYRQFA LDDQFSAKIF DRYLNMLDYS HNVLLASDVA QFADKKYSLD KELKSGQLDT PFALFNLAQK RRFERYQYAL SVLDRPMVFT GNDTIDIDRA KAPWPSSEAE LNALWDAKVK YDQLNLKLAG KTDKEIKETL TKRYQSAIKR LTQSNSEDVF QLIMNAFARE IDPHTNYLSP RNTEQFNTEM SLSLEGIGAV LQMDDDYTLI NSMVPGGPAA KSKTIAVGDR VIGVGQAGKP MVDVIGWRLD DVVALIKGPK GSKVRLEILP AGKGTKPRTV TLTRERIRLE DRAVKMSVKT IGKERVGVLD IPGFYVGLTE DVKVQLQKLE KQNVSSIIID LRSNGGGALT EAVSLSGLFI PSGPVVQVRD NNGKVREDSD TDGVVYYKGP LVVLVDRYSA SASEIFAAAM QDYGRALIVG EPTFGKGTVQ QYRSLNRIYD QMLRPEWPAL GSLQYTIQKF YRVDGGSTQR KGVTPDIVMP TGVDPAETGE NFEDNALPWD RINAASYTKT GDLKPWEPEL LKNHAERIAA NPEFQYIQKD IERYKALKDK RNIVSLNYAQ REKENRDDDM TRLTRLNERF IREGKKPLKS LDDLPKDYQE PDPYLDETVN IALDLAHKEK AQPQIASRPV AAATAGATVK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACAAATTTGTCAGACTAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTACCGTTGCCCCAGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008