Clone

Target

IDP00032  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

281  

Reference Sequence Used

MENKSAVQLY LELLKKTILF EIWLEYEPYL PASLHISKEL SYEPVTVPLP LFLKQYVENH NLKIVNPNVS NNERQGGKDW PRAAHSMVGR KRMNQLQEAM ETVVKENIEG DFIETGVWRV GSCIFMNGFL QANNITDRTV WVADSFEGLS APNLEQYPKD YGDYLHTFDY LRVSLETVQE NFRKYDLLND QVKFLKGWFK DTLPSAPIEK IAIARLDGDM YESTMDSLVN LYDKVSIGGF IIIDDYGLIT CAEAVTDFRS ERNIKTPMTQ IDDFGIFWRK E  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MENKSAVQLY LELLKKTILF EIWLEYEPYL PASLHISKEL SYEPVTVPLP LFLKQYVENH NLKIVNPNVS NNERQGGKDW PRAAHSMVGR KRMNQLQEAM ETVVKENIEG DFIETGVWRV GSCIFMNGFL QANNITDRTV WVADSFEGLS APNLEQYPKD YGDYLHTFDY LRVSLETVQE NFRKYDLLND QVKFLKGWFK DTLPSAPIEK IAIARLDGDM YESTMDSLVN LYDKVSIGGF IIIDDYGLIT CAEAVTDFRS ERNIKTPMTQ IDDFGIFWRK E  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAAAATAAATCAGCTGTCC  

PCR Backward Primer

TTATCCACTTCCAATGCTATTCTTTTCTCCAAAAAATACC  

Shipped To Lab

ANL  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008