Clone

Target

IDP00033  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

795  

Reference Sequence Used

MNKKPFKVLS SIALTAVLGL SFGAGGQSVY AETPVNKTAT SPVDDHLIPE ERLADALKKR GVIDSKASEK ETKKAVEKYV ENKKGENPGK EVTNGDPLTK EASDFVKKVK DAKADTKEKL DKPATGTPAA TGPVRGGLNG KVPTSPAKQK AYNGDVRKDK VLVLLVEYAD FKHNNIDKEP GYMYSEDFNK EHYEKMLFGD EPFTLDDGSK IETFKQYYEE QSGGSYTVDG TVTKWLTVPG KAADYGADAA TGHDNKGPKG PRDLVKDALK AAVDSGLDLS EFDQFDQYDV NGDGNKNQPD GLIDHLMIIH AGVGQEAGGG KLGDDAIWSH RWTVGPKPFP IEGTQAKVPY WGGKMAAFDY TIEPEDGAVG VFAHEYGHDL GLPDEYDTQY SGHGEPVQAW SIMSGGSWAG KIAGTTPTSF SPQNKEFFQK TIGGNWANIV EVDYEKLNKG IGLATYLDQS VTKTNRPGMI RVNLPDKDIK TIDPAFGKQY YYSTKGDDLH TKLETPLFDL TNATTAKFDF KSLYEIEAEY DFLEVHAVTE DGQQTLIERL GEKANNGNAD STNGKWIDKS YDLSQFKGKK VKLTFDYITD GGLALNGFLL DNASLTVDGK VVFSDDAEGT PQFKLDGFAV SNGTEKKSHN YYVEWRNYAG SDNALKFARG PEYNTGMVVW YADSAYTDNW VGVHPGHGFL GVVDSHPEAI VGTLNGKPTV ESSTRFQIAD AAFSFDKTPA WKVVSPTRGT YTYNGLAGVP KFDDSKTYIN QQIPDAGRIL PNLGLKFEVV GQADDNSAGA VRLYR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACAAGAAACCGTTCAAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGATATAAGCGAACAGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008