Clone

Target

IDP00049  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

512  

Reference Sequence Used

MIFLNIKKNT KRRKFLACLL VSLCTIHYSS ISFAETQAGN ATGAIKNASD INTGIANLKY DSRDILAVNG DKVESFIPKE SINSNGKFVV VEREKKSLTT SPVDILIIDS VVNRTYPGAV QLANKAFADN QPSLLVAKRK PLNISIDLPG MRKENTITVQ NPTYGNVAGA VDDLVSTWNE KYSTTHTLPA RMQYTESMVY SKSQIASALN VNAKYLDNSL NIDFNAVANG EKKVMVAAYK QIFYTVSAEL PNNPSDLFDN SVTFDELTRK GVSNSAPPVM VSNVAYGRTV YVKLETTSKS KDVQAAFKAL LKNNSVETSG QYKDIFEEST FTAVVLGGDA KEHNKVVTKD FNEIRNIIKD NAELSFKNPA YPISYTSTFL KDNATAAVHN NTDYIETTTT EYSSAKMTLD HYGAYVAQFD VSWDEFTFDQ NGKEVLTHKT WEGSGKDKTA HYSTVIPLPP NSKNIKIVAR ECTGLAWEWW RTIINEQNVP LTNEIKVSIG GTTLYPTATI SH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTTTTCTGAATATTAAGAAAAA  

PCR Backward Primer

TTATCCACTTCCAATGCTAATGACTAATAGTAGCAGTTG  

Shipped To Lab

ANL  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008