Clone

Target

IDP00201  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

727  

Reference Sequence Used

MIISPPFLRA KMSSQPDEEW VSSMMPADPQ RGYPISNSQA WHGGIHIKHT DHTGVPEQVR AIADGTVFSV RQPSLQKRDL LPLNYNGPTD CGYVLIKHET EIGSDEGGKV AYWSLYMHMK SIGSTVSPGS VVYRKDPLGT VGMVDGQNAI HFQIFCDDAN IKKLTGRETP ELDLANNGRT DVVYGDIHFY LPAGTPVYDS MPKDNTPVSL MANGPVPRTK SDLFVTMRFH QGSCTMTTLH KAVFSSMYLE VGEPLTDADG ADYEYNLYSK ALTLYPNSPS AGYELLRFGR VINTDNETLS PAGAPLWRTI NYPEGKGVVN LAAASVKVFS DADFPHWMGW QLVDDDTDTN SQCNSPTITL RCKTGVDLSG MICHFPLEWD KTTVDNRFQW LAKENDVLPE PMELCDLGPL TDHAKALCLA ENPLPSGRVW HFEPTRFIEH FRKCGWLSNK EMKQLIPTKA LSNGQWQTIP DRYGDTSVLA RHYSRINKVL RKYLINTPFR MACFFGNAIQ ETAWLTTTHE GYVYTERNQR TRAIIRHYNI WYYPWYGRGF LQLTSPGNYY KYFKYRGYTY DETVKIKLEN EYNRLYRNGS IRYSENHLDD TSNPELSAEI LNWRNSLETG DYEPADSAGF YWCTTQMAKY ADPEHILERQ VSANHIYYRS PSFWQASATV NLPGARQRTN YSGLNGFIER CYVYGSALAV LTELKFPDSQ GTYSLEKPEE NNLRRTL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATAATCAGCCCTCCTTTC  

PCR Backward Primer

TTATCCACTTCCAATGTCATAATGTTCTCCTTAAATTATTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011