Clone

Target

IDP00204  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

450  

Reference Sequence Used

MNSPVSPDYA IMNRAIFYKT IRYTDDILCR ATLYPMKIHA IQRPLSTARA QRCCECDALF TLPPLGGNQT AYCPRCNAQI TSGRDWSLTR LTAMACAMLL LIPFAFTEPL ISIRLLGTRI DASLLAGIWQ MSRQGDPITA SMVAFCVLAA PVTLTFSILY LRIGSRIGIN LRPILLILER LKEWVMLDIY LIGMAVACIK VKEYADIMPG TGLIAYLTLT LLSILTLVHL NLEQLWERFY PQEQPPGPQE TLRVCLSCHY TGHPDSHGRC PRCHTPLRHR RRHSLQKTWA ALIAAIVLLL PANLLPISIV YANGARIEDT IFSGVVSLAS SGNFPIAAVV FIASVLVPFT KVIVLITLLL SIHLKTQHSL KTRMRLLRLI TWIGRWSMLD LFVIALMMSL INRDQLFSFT MGPAAFYFGS AVILTILAVE WLDSRLIWDA YATGNTEYTD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATTCACCTGTTTCGCCC  

PCR Backward Primer

TTATCCACTTCCAATGTCAGTCGGTGTACTCGGTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011