Clone

Target

IDP00211  

Clone Suffix

1 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

211  

Reference Sequence Used

MANSDTQMKR PYPPLSFVNE FRPHIELVPA TEVLEWVNSQ ILSDDGELHN PDHGHLIDAD IKIMWASSAF EKQGRTVLGQ AEQVAMRAGG WQKARMEQQM YEWFGDVPTF IITLAADYCA QCSDLEFCAL IEHELYHISH ATDEFGAPKF NKEGQPVLKL RGHDIEEFVG VVRRYGASVE VQEMIDAANK PAEVAHLNVA RACGTCLLKL A  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MANSDTQMKR PYPPLSFVNE FRPHIELVPA TEVLEWVNSQ ILSDDGELHN PDHGHLIDAD IKIMWASSAF EKQGRTVLGQ AEQVAMRAGG WQKARMEQQM YEWFGDVPTF IITLAADYCA QCSDLEFCAL IEHELYHISH ATDEFGAPKF NKEGQPVLKL RGHDIEEFVG VVRRYGASVE VQEMIDAANK PAEVAHLNVA RACGTCLLKL A  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTAATTCAGATACACAAAT  

PCR Backward Primer

TTATCCACTTCCAATGTTAAGCCAGTTTTAATAGGCATG  

Shipped To Lab

ANL  

Shipped Date

Sep 29, 2011  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011