Clone

Target

IDP04055  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

836  

Reference Sequence Used

MKFKQPALLL FIAGVVHCAN AHTYTFDASM LGDAAKGVDM SLFNQGVQQP GTYRVDVMVN GKRVDTRDVV FKLEKDGQGT PVLAPCLTVS QLSRYGVKTE DYPQLWKAAK PPDECADLTA IPQAKAVLDI NNQQLQLSIP QLALRPEFKG IAPEDLWDDG IPAFLMNYSA RTTQTDYKMD MVGRDNSSWV QLQPGINIGA WRVRNATSWQ RSSQLSGKWQ AAYTYAERGL YSLKSRLTLG QKTSQGEIFD SVPFTGVMLA SDDNMVPYSE RQFAPVVRGI ARTQARVEVK QNGYTIYNTT VAPGPFALRD LSVTDSSGDL HVTVWEADGS TQMFVVPYQT PAIALHQGYL KYSLLAGRYR SSDSATDKAQ IAQATLMYGL PWNLTAYGGI QSATHYQAAL LGLGGSLGRW GSLSVDGSDT HSQRQGEAVQ QGASWRLRYS NQLTATGTNF FLTRWQYASQ GYNTLSDVLD SYRHNGNRLW SWRENLQPSS RTTLMLSQSW GRHLGNLSLT GSRTDWRNRP GHDDSYGLSW GTSIGGGSLS LNWNQNRTLW RNGAHRKENI TSLWFSMPLS RWTGNNVSAS WQMTSPSHGG QTQQVGVNGE AFSQQLDWEV RQSYRADAPP GGGNNSALHL AWNGDYGLLG GDYSYSRAMR QMGVNIAGGI VIHHHGVTLG QPLQGSVALV EAPGASGVPV GGWPGVKTDF RGDTTVGNLN VYQENTVSLD PSRLPDDAEV TQTDVRVVPT EGAVVEAKFH TRIGARALMT LKREDGSAIP FGAQVTVNGQ DGSAALVDTD SQVYLTGLAD KGELTVKWGA QQCRVNYRLP AHKGIAGLYQ MSGLCR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGTTCAAACAACCTGCC  

PCR Backward Primer

TTATCCACTTCCAATGCTATCTGCAGAGACCGCTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011