Clone

Target

IDP04367  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

622  

Reference Sequence Used

MKITELLKRD TVIMNLTASN KEAVIDELVE KLNGANRLNS KAEFKEAILK RESQSTTGIG EGIAIPHAKT KAVKQPAICF GRSVSGINYE SLDGQPAHLF FMIAASEGAN NTHLETLSRL STLLMDEGFR KQLLEAKDEE ELLRLFDEKE NEKENEKEEE VEVAQPEGNE PYVLAVTACP TGIAHTYMAA DSLKAKAAEL GIAIKVETNG STGIKNGLTK EDIERATAII VAADKQVEMN RFAGKHVIQV PVADGIRKTE KLLNRAVKQD APIFKGIKED GKTESTEKEK GLGIYKHLMN GVSNMLPFVV GGGILIALAF SFGGIKAEGP LAELFMSIGG GGTGAFLFLV PILAGFIASS IADRPGFMPG VVGGFLAAHA NAGFLGGLIA GFLAGYVVLG LKRLFSGLPV QLEGIKPVLL YPVFGLLITG VVMQKVVIPP VVALNEMLTG WLNGLNGTNA ILLGLILGAM MAIDMGGPIN KAAFTFGIAA IEAQNFGVHS AVMAGGMVPP LAIAFATTFF KSKFTEAERK SGLTNYIMGA SFITEGAIPF AAADPVRVIV SCVVGSSIAG ALSMLFQITL PAPHGGLFVI ALVNKPVLYI FSILIGAVVS ALMMGIWKKK VK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATTACAGAACTATTAAAAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTAACTTTCTTTTTCCAAATC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011