Clone

Target
IDP04540  
Clone Suffix
1 The clone is not available in BEIR.
Experiment Start Date
Oct 17, 2011  
Experiment End Date
Oct 17, 2011  
Person
James Winsor  
Lab
Northwestern University  
Protocol ID
NU_native_expression  
Status
success  
Vector
pMCSG 7  
Sequence Start
1  
Sequence End
516  
Reference Sequence Used
 
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
EDKLSYMDED VRNTLKETAF SISEIPFIQE DLSNGEINSR IQEYTKHFIE AINDVDIIVV ADMRGVKYSH LDEKQIGQVF VNEDKKEVLT QGSSYYSLMK GSMGETLRWF QPVMYNGKQV GFIMVGKYYN EIQLLTHK  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Oct 11, 2011  
Secondary Shipped To Lab
NU  
Secondary Shipped Date
Oct 11, 2011  
Inserted
Oct 04, 2011  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-11-09 2011-11-09 James Winsor Northwestern University NU_SelMet_expression success E. coli SeMet IPTG production
2011-07-25 2011-08-02 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay