Clone

Target

IDP00173  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

486  

Reference Sequence Used

MTRIKLSYFT IGLVALLLAL ALWPNIPWRN GQEGQLDQIK ARGELRVSTI SSPLIYSTEK DTPSGFDYEL AKRFADYLGV KLVIIPHHNI DDLFDALDND DTDLLAAGLI YNRERLNRAR TGPAYYSVSQ QLVYRLGSPR PKSFSDLKGQ VVVASGSAHM TTLKRLKQTK YPELNWSSSV DKSGKELLEQ VAEGKLDYTL GDSATIALLQ RIHPQLAVAF DVTDEEPVTW YFKQSDDDSL YAAMLDFYSE MVEDGSLARL EEKYLGHVGS FDYVDTKTFL SAIDNVLPSY QHLFEKHAGD IDWKLLAVIA YQESHWNPQA TSPTGVRGLM MLTRATADGL GVKDRVDPEE SIRGGAIYLQ RLMKKLPETI PEDERIWFAL AAYNLGYGHM LDARRLTKNQ NGNPDSWVDV KMRLPMLSQK RYYPSTTYGY ARGHEAYNYV ENIRRYQVSL VGYLQEKEKK AAQHAAIEAE LGKSNPVVGP GWSIGD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACACGCATAAAATTAAGTTAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTCACCGATTGACCAACC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008