Clone

Target

IDP00418  

Clone Suffix

4 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Nov 02, 2011  

Experiment End Date

Nov 02, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

437  

Reference Sequence Used

MTQQEYQNRR QALLAKMAPG SAAIIFAAPE ATRSADSEYP YRQNSDFSYL TGFNEPEAVL ILVKSDETHN HSVLFNRIRD LTAEIWFGRR LGQEAAPTKL AVDRALPFDE INEQLYLLLN RLDVIYHAQG QYAYADNIVF AALEKLRHGF RKNLRAPATL TDWRPWLHEM RLFKSAEEIA VLRRAGEISA LAHTRAMEKC RPGMFEYQLE GEILHEFTRH GARYPAYNTI VGGGENGCIL HYTENECELR DGDLVLIDAG CEYRGYAGDI TRTFPVNGKF TPAQRAVYDI VLAAINKSLT LFRPGTSIRE VTEEVVRIMV VGLVELGILK GDIEQLIAEQ AHRPFFMHGL SHWLGMDVHD VGDYGSSDRG RILEPGMVLT VEPGLYIAPD ADVPPQYRGI GIRIEDDIVI TATGNENLTA SVVKDPDDIE ALMALNH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

LTLRRSLSHD SARIPEPPSG TVGENGPWQC CYYFCRTRSH TQCRF*ISLS AE**F*LSDG LQRARSGVDS G*KR*DS*PQ RAV*PDPGFN R*DLVWSSVR ARGRPHETGS RSRITFR*NQ RAALFAA*SP GCDLSRSRAI CLRR*YCFCC TGKITSWFS* KSPSASHVNR LAALVA*NAS V*ISRRDRRA APRR*NQRTG PYPCDGKMPP RYV*ISIGRG NSA*IYPPWR ALSSVQHHRW WG*KRLHFAL YRE*V*AAGR GFGPYRRGL* IPWLCR*YHS HFPGKWQIYP RSAGGL*HRS GGYQ*IADVV PPRYQHP*GH GRSGADHGRR FGGVGYSER* YRTVDR*TSP SAILYAWPKP LAGDGCP*RR RLR*Q*PWPY P*TGHGINRG TGLVHCPRCR CPAAIPGHWY SY*R*HCDYR HG*RKLDRER G*RP**H*SI DGIESRGEPV LP  

PCR Forward Primer

GTCTCTCCCATGACTCAGCAAGAATACCAG  

PCR Backward Primer

GGTTCTCCCCACGGTGATTCAATGCCATCAATGC  

Shipped To Lab

ANL  

Shipped Date

Apr 30, 2012  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012