Clone

Target
IDP04376  
Clone Suffix
104
Experiment Start Date
Feb 27, 2012  
Experiment End Date
Mar 01, 2012  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
382  
Reference Sequence Used
MARILKTAYT FDDVLLVPNK SEVLPNEVSL KTQLTKKIQL NIPLMSASMD TVTESKMAIA MAREGGIGII HKNMTIEDQA REVDRVKRQE SGGIKDIEKA KAFPNAAKDE KGRLLCGASI GVTNDMMERV DAVVKAKVDV IVLDTAHGHS KGVIEGVKRI KAKYPELQVI AGNIATPEAV RDLAEAGADC VKVGIGPGSI CTTRIVAGVG VPQLTAVMDC AEEGKKLGIP VIADGGLKYS GDIVKALAAG ACAAMMGSIF AGCEEAPGAI EIYQGRSYKV YRGMGSLGAM AKGSSDRYFQ NGTKKFVPEG VEGRIAYKGH LADTIYQLIG GIKSGMGYLG APTLENLYEN ANFVVQTSAG FRESHPHDIN ITKEAPNYSV NQ  
PCR Forward Primer
TACTTCCAATCCAATGCCATGGCAAGAATATTAAAAACAGCATATACATTTGATGAT  
PCR Backward Primer
TTATCCACTTCCAATGTTATTGGTTAACACTGTAGTTTGGTGCTTCC  
Shipped To Lab
ANL  
Shipped Date
Mar 01, 2012  
Inserted
Apr 12, 2012  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-04-18 2012-04-20 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 0.5 mM IPTG production
2012-03-05 2012-03-05 Minyi Gu Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2012-03-14 2012-03-16 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 0.5 mM IPTG production