Clone

Target
IDP00217  
Clone Suffix
7
Experiment Start Date
Jun 29, 2012  
Experiment End Date
Jul 03, 2012  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
21  
Sequence End
278  
Reference Sequence Used
LDKPDGSNSI EDQTALFFGK DDRTAVTNSR QWPWEAIGQV ETASGNPCTA TLISPRLVLT AGHCVLTPPG NIDQAVALRF ISDKGHWKYQ ITDLKTRVDA KLGQKLKADG DGWIVPPAAA AYDFALIQLT NAAPIPIKPL PLWEGTANEL TKALKLVNRK VTQAGYPLDN LNTLYKHEDC LVTGWAQQGV LAHQCDTLPG DSGSPLLLKN GNSWSLIAIQ SSAPAAKERY LADNRALSVT AINNRLKKLV NKIADTTQ  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGCTAGATAAACCGGACGGAAG  
PCR Backward Primer
TTATCCACTTCCAATGTTACTGAGTTGTATCGGCTATC  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-07-05 2012-07-11 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-07-24 2012-07-24 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay