Clone

Target

IDP00268  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Jun 29, 2012  

Experiment End Date

Jul 03, 2012  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

315  

Sequence End

900  

Reference Sequence Used

GSASSMVNGV VKLLTKPWDV IPMVTQLAMT DTTPFGQQRV FKEKVDTRTP QPKPGTRMVM TTTANWLWAL LGKKKNPRLC TREEFISKVR SNAAIGAVFQ EEQGWTSASE AVNDSRFWEL VDKERALHQE GKCESCVYNM MGKREKKLGE FGRAKGSRAI WYMWLGARFL EFEALGFLNE DHWFGRENSW SGVEGEGLHR LGYILEDIDK KDGDLIYADD TAGWDTRITE DDLLNEELIT EQMAPHHKIL AKAIFKLTYQ NKVVKVLRPT PKGAVMDIIS RKDQRGSGQV GTYGLNTFTN MEVQLIRQME AEGVITQDDM HNPKGLKERV EKWLKECGVD RLKRMAISGD DCVVKPLDER FSTSLLFLND MGKVRKDIPQ WEPSKGWKNW QEVPFCSHHF HKIFMKDGRS LVVPCRNQDE LIGRARISQG AGWSLRETAC LGKAYAQMWS LMYFHRRDLR LASMAICSAV PTEWFPTSRT TWSIHAHHQW MTTEDMLKVW NRVWIEDNPN MTDKTPVHSW EDIPYLGKRE DLWCGSLIGL SSRATWAKNI HTAITQVRNL IGKEEYVDYM PVMKRYSAPF ESEGVL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGGCTCAGCATCCTCCATGG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGAACTCCTTCACTCTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 21, 2012