Clone

Target

IDP04376  

Clone Suffix

109

Sequence Comments

IDP04376.1-484.F.Q89_R215delinsSGG.pMCSG50  

Experiment Start Date

Feb 27, 2012  

Experiment End Date

Sep 10, 2012  

Person

Minyi Gu  

Lab

Argonne National Laboratory  

Protocol ID

standard cloning_ANL  

Status

failed  

Vector

pMCSG50  

Sequence Start

1  

Sequence End

484  

Reference Sequence Used

MARILKTAYT FDDVLLVPNK SEVLPNEVSL KTQLTKKIQL NIPLMSASMD TVTESKMAIA MAREGGIGII HKNMTIEDQA REVDRVKRQE NGVITDPIFL SEDHTVREAL DLMAQYRISG VPVTREGKLV GIITNRDIVF ETNYDKKVSE VMTKSPLVTA KEGTTLTEAL EILKQHKIEK LPLVDDENNL KGLITIKDIE KAKAFPNAAK DEKGRLLCGA SIGVTNDMME RVDAVVKAKV DVIVLDTAHG HSKGVIEGVK RIKAKYPELQ VIAGNIATPE AVRDLAEAGA DCVKVGIGPG SICTTRIVAG VGVPQLTAVM DCAEEGKKLG IPVIADGGLK YSGDIVKALA AGACAAMMGS IFAGCEEAPG AIEIYQGRSY KVYRGMGSLG AMAKGSSDRY FQNGTKKFVP EGVEGRIAYK GHLADTIYQL IGGIKSGMGY LGAPTLENLY ENANFVVQTS AGFRESHPHD INITKEAPNY SVNQ  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCCATGGCAAGAATATTAAAAACAGCATATACATTTGATGAT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGGTTAACACTGTAGTTTGGTGCTTCC  

Shipped To Lab

ANL  

Shipped Date

Sep 10, 2012  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 27, 2012