Clone

Target
IDP00892  
Clone Suffix
4
Experiment Start Date
Mar 04, 2013  
Experiment End Date
Mar 04, 2013  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
139  
Reference Sequence Used
MKKGTVLNSE ISSVISRLGH TDTLVVCDAG LPIPNSTARI DMALTQGVPS FMQVVDVVTR EMQVEAAILA TEIKQQNPQL HETLLTHLEQ LQQHQGNTIK ISYTTHEQFK KLTADSQAVI RSGECSPYAN VILCAGVTF  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAGAAAGGCACCGTACTC  
PCR Backward Primer
TTATCCACTTCCAATGTCAGAAAGTGACGCCAGCG  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2013-04-04 2013-04-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay