Clone

Target

IDP04007  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Nov 20, 2012  

Experiment End Date

Nov 27, 2012  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG28  

Sequence Start

25  

Sequence End

139  

Reference Sequence Used

FIVYELDTDY DYVLEDYQAE ALQTLLSQIR QLDSPESAKT ALRGYAEHIH KTVDLLPLTE LPPEVQQHFS SEAAHSPIFH DDDRNLWLQL DSNEQIYLLK PDSHSPVYQA IDRAD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCTTTATTGTCTATGAATTAGATACC  

PCR Backward Primer

GGTTCTCCCCAGCATCGGCGCGATCTATGGCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 21, 2013