Clone

Target

IDP01173  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

505  

Reference Sequence Used

MTVATYEVEK QFLTYVKKIQ NYGEALSLMF WDLRTGAPKK GVDQRSEVIG MLSSEVFAMS TSDEMGNYLT ELEALISEDK LSETTKKMVE ECRKEYDRNK KIPQAEYEAY VKLEAKAESV WEEAREKSDF EMFRPYLEQI VEFKKKFITY WGYETYKYNT LLDMYEPGIT VEVLDHVFGQ LRERIVPLVK EISESQKRLK TSALSEHFSK EKQKNFTLEL LKQLNYDFEA GRLDETVHPF EITLNRGDVR ITTRYDEKDF RMAVFGTIHE CGHAVYEQNI AEKFEGTPLC SGTSMGIHES QSLFFENFIG RNKSFWKKNY DLLKEYSDGQ FNDISVDEFY DAINESKPSF IRIEADELTY PLHVMVRYEL EKELFDGTLQ VKDLPAAWND KMEAYLGIRP ANDAQGVLQD VHWAGGSFGY FPSYALGYMY AAQFKQRMVK DIPNFDALLE EGNVTPIREW LTENIHQYGK TKKPLEILED VTGEGLNANY LADYLEAKYR EIYEL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACAGTAGCTACATATGAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTATAACTCGTAAATCTCTCTATA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008