Clone

Target

IDP00532  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

383  

Reference Sequence Used

MSSSCIEDQS VQESPWFRIV QDMLNQADIE INGSRPFDIR VNNPDFFKRV LQGGSLALGE SYMDGWWECD RLDIFFQRVL QAGLEHKLPR HFKDTLRIAA ARVINLQSKR RAWIVGKEHY DLGNDLFTQI LDPNMQYSCA YWKEATSLEQ AQEHKLQMIC EKLQLAPGMK LLDIGCGWGG LAAYAARHFG VSVSGVTISV EQQKLAQQRC EGLDVSILLQ DYRDLNEQFD RIVSVGMFEH VGPKNYSTYF KVVNRNLKPD GLFLLHTIGA NRTNMNVDPW INKYIFPNGC LPSVQHIAQA SEPYFVMEDW HNFGADYDRT LMAWYERFQA AWPNLSANYS QRFERMFSYY LNACAGAFRA RDIQLWQVLF SPNGVNGGIR VPR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTTCATCCTGTATAGAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGAGGGACGCGAATC  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008