Clone

Target

IDP00843  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

1016  

Reference Sequence Used

MEEGYRLKFI FIGNQNNNIK NKNFSNPHNI LFDSKKDIIL TQDLCEEFLN LNINKQDHAI ELLKKELSPL LFEDSLSYLK EEFINEKLEF NISNLASRYT ANNDVDTINN KIIEGISITN NFKYTNISYL KELKGYIEND ILDKMKSKYA KNIYLNFKKI FSNLEQSVNN YLELEEEFEE KKKYLSEIYE LIDEINIDPY IFLTEHNECN IYKISENEKL ELQTYMSKIE KVLLKYQTYL KETCKECLFY PYLLVQGEAG IGKSHLLAHL SKKLRDENHI IYLFLGQFFT KNEDPWHQIL NDLEVTNSVD NFLRSISNKA KETKKRAFII IDALNEGEGK RLWGNYFQSF INHIKKYSNI ALIFSIRTPF EDVILPKNAI QDNNIVVFQH EGFSKEENYN PIVSFCDFYG LELPKLPILN PEFNNPLFLK LMCEYCVNKF KEFDQTISVA ELFTNVLKTV NINLSKEDKF DFDKNINVVQ KVIKGLVELM NDSEFNQLNY EESYTVVNNI AKEYVQKSNR FLEALIDENI LIKNTGYKGE MIIYFSYERM GDYFLSEYLL EKYRNVDKRD LVTKLQSDEK VTRYFQKEDD LSYNRGLINE LFIKLANEFN IELFEVFPQF KNNYNMIYSF INSLVWRKDG SISKHTKCYI SDNVIPYDAF RNNFLDVLLI KMPQKNHPLN IWALHKLLKQ CNLGKRDFLW TQYISINNEK VFEIINWLFS NYKKLDEETA EKYMIFLTWI FSATNNKLRD LGTKSLVKLF KTFPTKIIGL LKLFENNNDP YIVERLYASV LGATLRIDIC EIHIEIANYI YEEIFDKEMV YPHILMRDYA RQTIEYISLS KDISNINLEK IRPPYKSNWY KKEYSNLNID DYIKSLKNKL DSHLHFSIDK IKNSMTTEYG RGTGAYGDFG RYVFGYAVRN WVKGFKSDQD LSNIALMRIF EMGYDAKLHG EFDMWVNRYD NFNNSIERIS KKYQWIAYYE ILAKLVDKFP DVQYSGLWDD YIRDIDPTLL LLEIDK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAAGAAGGTTATCGATTAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTATCAATTTCAAGTAATAGTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008