Clone

Target
IDP04007  
Clone Suffix
5
Experiment Start Date
May 02, 2013  
Experiment End Date
May 08, 2013  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG28  
Sequence Start
25  
Sequence End
139  
Reference Sequence Used
MFIVYELDTD YDYVLEDYQA EALQTLLSQI RQLDSPESAK TALRGYAEHI HKTVDLLPLT ELPPEVQQHF SSEAAHSPIF HDDDRNLWLQ LDSNEQIYLL KPDSHSPVYQ AIDRAD  
Reference Sequence Variance
MATCH  
PCR Forward Primer
GTCTCTCCCATGTTTATTGTCTATGAATTAGATA  
PCR Backward Primer
GGTTCTCCCCAGCATCGGCGCGATCTATGGCT  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2013-05-13 2013-05-16 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay