Clone

Target

IDP93935  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jan 13, 2014  

Experiment End Date

Jan 23, 2014  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

221  

Reference Sequence Used

MKDKPILGPD PQCKHPMAGF NQVCFIKNTT QNPNIIIGDY TYYDDPQDSE NFERNVLYHY PFIGDKLIIG KFCALAHGVK FIMNGANHKM SGLSTYPFNI FGNGWERVAP SRDELPYKGD THVGNDVWIG YDVLIMPGVT IGNGAIISSR SVVTRDVPAY SVVGGNPATL IKNRFSAEVI GKLQTIAWWD WPIDAISRNL HLIVAGDIEA LARAASEIEH T  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKDKPILGPD PQCKHPMAGF NQVCFIKNTT QNPNIIIGDY TYYDDPQDSE NFERNVLYHY PFIGDKLIIG KFCALAHGVK FIMNGANHKM SGLSTYPFNI FGNGWERVAP SRDELPYKGD THVGNDVWIG YDVLIMPGVT IGNGAIISSR SVVTRDVPAY SVVGGNPATL IKNRFSAEVI GKLQTIAWWD WPIDAISRNL HLIVAGDIEA LARAASEIEH T  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGATAAGCCAATATTAGG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGGTGTGTTCAATCTCGCT  

Shipped To Lab

UT  

Shipped Date

Mar 11, 2014  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 14, 2014