Clone

Target

IDP01230  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

170  

Reference Sequence Used

MDFKQHIAIV PDYPKEGIVF KDITPLMNDG KAYKAATDAI VEYAKERDID LVVGPEARGF IIGCPVSYAL EVGFAPVRKL GKLPREVITV DYGKEYGKDV LTIHKDAIKP GQRVLITDDL LATGGTIEAT IKLVEELGGV VAGIAFLVEL TYLDGRKMLD GYDVLVLEKY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATTTCAAGCAACATATCG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTATTTTTCTAATACTAATACAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008