Clone

Target

IDP00268  

Clone Suffix

7

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

900  

Reference Sequence Used

GTGTTGETLG EKWKRQLNSL DRKEFEEYKR SGILEVDRTE AKSALKDGSK IKHAVSRGSS KIRWIVERGM VKPKGKVVDL GCGRGGWSYY MATLKNVTEV KGYTKGGPGH EEPIPMATYG WNLVKLHSGV DVFYKPTEQV DTLLCDIGES SSNPTIEEGR TLRVLKMVEP WLSSKPEFCI KVLNPYMPTV IEELEKLQRK HGGSLVRCPL SRNSTHEMYW VSGVSGNIVS SVNTTSKMLL NRFTTRHRKP TYEKDVDLGA GTRSVSTETE KPDMTIIGRR LQRLQEEHKE TWHYDQENPY RTWAYHGSYE APSTGSASSM VNGVVKLLTK PWDVIPMVTQ LAMTDTTPFG QQRVFKEKVD TRTPQPKPGT RMVMTTTANW LWALLGKKKN PRLCTREEFI SKVRSNAAIG AVFQEEQGWT SASEAVNDSR FWELVDKERA LHQEGKCESC VYNMMGKREK KLGEFGRAKG SRAIWYMWLG ARFLEFEALG FLNEDHWFGR ENSWSGVEGE GLHRLGYILE DIDKKDGDLI YADDTAGWDT RITEDDLLNE ELITEQMAPH HKILAKAIFK LTYQNKVVKV LRPTPKGAVM DIISRKDQRG SGQVGTYGLN TFTNMEVQLI RQMEAEGVIT QDDMHNPKGL KERVEKWLKE CGVDRLKRMA ISGDDCVVKP LDERFSTSLL FLNDMGKVRK DIPQWEPSKG WKNWQEVPFC SHHFHKIFMK DGRSLVVPCR NQDELIGRAR ISQGAGWSLR ETACLGKAYA QMWSLMYFHR RDLRLASMAI CSAVPTEWFP TSRTTWSIHA HHQWMTTEDM LKVWNRVWIE DNPNMTDKTP VHSWEDIPYL GKREDLWCGS LIGLSSRATW AKNIHTAITQ VRNLIGKEEY VDYMPVMKRY SAPFESEGVL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGGAACTGGGACCACAGGAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGAACTCCTTCACTCTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014