Clone

Target

IDP95255  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Oct 02, 2014  

Experiment End Date

Oct 02, 2014  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

654  

Reference Sequence Used

MIEQKSPAQA EIEILKSRLV LGTVIKDLHL NIQVSSTENT LTHRLLSDAD YKTEYTTKSV IFKDGLKSFD IRQFEIPAYY LDKNLLLDFD KQSLRLTDPV TEEVILTVPL NQVNHVAGPH GLWKVAIFTK DQFDATYNIK SLSLPIAVNA ISANYAVAER GKLTGVLGLN YQGQDKEHIT KVLNAILATY SAQNIERRSA ESAQTLKFLD EQLPDLKKQL DDAERQFNKF RQQYNTVDVT KESELYLTQS ITLETKKAEL EQKQAEMAAK YTAEHPAMRE INGQLAAINK QIGELNSTLK QLPDVQRQYL QLYREVEVKT QLYTALLNSY QQLRIAKAGE IGNVRIVDTA VEPVEPIKPK KLLVLILSVF VGGFIGALIA LLRNMLRTGI KDSGQIENEM DLPVYATVPR SPIQESRIKI LKKKKSIPIL AVKNSDDIAI ESLRSIRTAI HFALANAKNN IIMIAGPSPE VGKSFISTNL ATIFAQGNKR VLLIDADMRR GYMHKYFDVD VKPGLSELLS GQADLQKVLH KTQVANLDVI TRGKSPTNPS EILSSNQFKE LLEQLQSQYD HIIIDTPPVL AVTDGIIISQ YTGVNLIVAR YAKSQMKELE LTVNRFEQAG VKVNGFILND IQRASAGYGY GYNYAYAYKA QKED  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTGAGCAAAAATCACCGG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTCTTCTTTTTGTGCCTTAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014