Clone

Target

IDP00216  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

499  

Reference Sequence Used

MTTAYNHLCT AFTRLSRFEH LSAIAGWDMQ TMMPAKGNLA RSEAMAELNV LQHQILTAPQ IGEWLKQAEQ ELLDEQSIDE LKLANLREMH RHYHNAVLLP ESLVEAKSLA GARCEHAWRQ QRIANDWAGF AENLREVVKL SREEAKIRAE AAGTSGYDAL LNLYEPGTNS ADIDRIFGDL KQWLPALLQK VTTKQQSSEP CLIPQGPFDL EKQRQLGLSV MKVLGFDFNG GRVDVSVHPF CGGVPQDVRI TTRYNNQEFL SALMGIVHET GHARYEQNLP REWLGQPIAH ARSTAIHESQ SLLFEMQLAR SNEFLQVIHP LVIQQFGEQP AFAEHNFIAL NQRVKTGLIR VDADEVSYPA HVILRYEIEK ALIGGEIDVE DIPALWNEKM QQYLGINTEG DYRNGCMQDI HWTDGAFGYF PTYTLGAMYA AQLFHAARSA IPALDSHIAN GNLAPLLNWL QQNIWQHGSR YPTAELITKA TGEPLNPRYF RQHLERRYL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACAACCGCTTATAACCATC  

PCR Backward Primer

TTATCCACTTCCAATGTCAGAGATAACGGCGTTCTAA  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008