Clone

Target

IDP00422  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

432  

Reference Sequence Used

MGKNVVVLGT QWGDEGKGKV VDLLTERAKY VVRYQGGHNA GHTLVINGEK TVLHLIPSGI LRENVISIIG NGVVLAPDAL MKEMTELEAR GVPVRERLLL SEACPLILPY HVALDNAREK ARGAKAIGTT GRGIGPAYED KVARRGLRVS DLFNKETFAI KLKEIVEYHN FQLVHYYKEA AVDYQKVLDD VLAIADILTA MVVDVSELLD NARKQGELIM FEGAQGTLLD IDHGTYPYVT SSNTTAGGVA TGSGLGPRYV DYVLGIVKAY STRVGAGPFP TELNDETGEF LRKQGNEYGA TTGRSRRTGW LDIVAVRRAV QINSLSGFCM TKLDVLDGLK EVKLCVGYRM PDGREVDTTP LAAEGWEGIE PIYETMPGWS ETTFGVKEHS KLPQAALNYI QRVEELTGVP IDIISTGPDR DETMILRDPF DA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGTAAGAACGTCGTCGT  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCATCAAATGGATCACGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008