Clone

Target

IDP00794  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

May 14, 2015  

Experiment End Date

May 14, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

28  

Sequence End

608  

Reference Sequence Used

ILVLRISYIM ITGHSNGQDL VMKANEKYLV KNAQQPERGK IYDRNGKVLA EDVERYKLVA VIDKKASANS KKPRHVVDKK ETAKKLSTVI NMKPEEIEKR LSQKKAFQIE FGRKGTNLTY QDKLKIEKMN LPGISLLPET ERFYPNGNFA SHLIGRAQKN PDTGELKGAL GVEKIFDSYL SGSKGSLRYI HDIWGYIAPN TKKEKQPKRG DDVHLTIDSN IQVFVEEALD GMVERYQPKD LFAVVMDAKT GEILAYSQRP TFNPETGKDF GKKWANDLYQ NTYEPGSTFK SYGLAAAIQE GAFDPDKKYK SGHRDIMGSR ISDWNRVGWG EIPMSLGFTY SSNTLMMHLQ DLVGADKMKS WYERFGFGKS TKGMFDGEAP GQIGWSNELQ QKTSSFGQST TVTPVQMLQA QSAFFNDGNM LKPWFVNSVE NPVSKRQFYK GQKQIAGKPI TKDTAEKVEK QLDLVVNSKK SHAANYRIDG YEVEGKTGTA QVAAPNGGGY VKGPNPYFVS FMGDAPKKNP KVIVYAGMSL AQKNDQEAYE LGVSKAFKPI MENTLKYLNV GKSKDDTSNA EYSKVPDVEG Q  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATATTGGTTTTAAGAATTTCATATAT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGACCTTCAACATCTGGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 16, 2015