Clone

Target
IDP95711  
Clone Suffix
2
Experiment Start Date
Mar 20, 2016  
Experiment End Date
Mar 20, 2016  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
115  
Sequence End
257  
Reference Sequence Used
IADAHKVRVQ KAQKTAMNKL MGQIGKPYRW GGTSPRTGFD CSGLVYYAYK DLVKFRIPRT ANEMYHLRDA APVNRGELQN GDLVFFRTQG RGTADHVGVY VGNGKFIQSP RSGQDIQITS LSEDYWVRHY VGARRVMTPK TIR  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
IADAHKVRVQ KAQKTAMNKL MGQIGKPYRW GGTSPRTGFD CSGLVYYAYK DLVKFRIPRT ANEMYHLRDA APVNRGELQN GDLVFFRTQG RGTADHVGVY VGNGKFIQSP RSGQDIQITS LSEDYWVRHY VGARRVMTPK TIR  
PCR Forward Primer
TACTTCCAATCCAATGCGATTGCCGACGCGCATAAGG  
PCR Backward Primer
TTATCCACTTCCAATGTTAGCGGATGGTTTTCGGCG  
Shipped To Lab
UT  
Shipped Date
May 02, 2016  
Inserted
May 05, 2016  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-04-06 2016-04-06 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2016-05-26 Joanna Sandoval University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2017-02-21 Christopher Mcchesney University of Calgary Expression_protocol_Toronto success E. coli LB none production