Clone

Target
IDP52512  
Clone Suffix
102
Sequence Comments
IDP52512.1-465.F..pMCSG68  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG68  
Sequence Start
1  
Sequence End
465  
Reference Sequence Used
MEKFIERLLE EDIKFEKDVN NGLSDINIFD ALNIETKENY HSKFIAYLID INKDHYQKNF AKVFLEKLGK SLVNTKFENL NIEDIKSVET EACIKDNRRI DILITLSDKR YIIIENKIYA KDQKNQLKDY INFVRKNIKN IKDCYKNILT IYLHQDECAS PSDYSLGNFT IKTNLIKDKN ENNVSYYLKM DYMWIKEWID ECIKIYEEKS TKDQKFILDI QNIIFTLNQY KSILQWYIAD EYTQRDDVLE FIFQDIIKMQ NLKNAMILYR YNKNKSELKN LNEEKYKKAK DIIQHKWSNI CEYIIEEFFD SFEHKEIKIG DITFIGNKIE ENRVNHGVFI FYPVDYKNES IYPCIYIYFK KKYYDIIGLT FEISNDDEED IENEKYKECL KLFKDVKEEN VRKYQNHYYC DKLINNEKLE GEYAFIYWLI ENQNSKKDFI QILNDFFIKK PIQEAYKGIN DILNS  
PCR Forward Primer
TACTTCCAATCCAATGCCATGGAAAAATTTATTGAAAGATTGCTTGAAGAAGATATTAAATTT  
PCR Backward Primer
TTATCCACTTCCAATGTTAAGAATTTAATATATCATTAATACCTTTATATGCTTCCTGTATA  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-26 2016-05-28 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production