Clone

Target
IDP63272  
Clone Suffix
108
Sequence Comments
IDP63272.5-395.P(399)..pMCSG73  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG73  
Sequence Start
5  
Sequence End
395  
Reference Sequence Used
ELPDEFGHFG PYGGMFVADT LVHALKQLEH AYTKYRNDQD FLSELHTELK DYVGRPNPLY HAVHLSKKIG GAQIYLKRED LNHTGAHKIN NTIGQALLAK RMGKTRVIAE TGAGQHGVAT ATVAAKFGFQ CVVYMGSEDI KRQSSNVYRM KLLGAEVVPV TSGSKTLKDA LNEALRDWVS HVDDTFYIIG TVAGPHPYPQ MVRDFQAIIG VEARAQHMEK TGRLPDALVA CVGGGSNAIG LFYPFLNDQS VMIYGVEAGG KGIETGEHSA SLIAGKPGVL HGNRTYLLCD EYGQVKDTHS VSAGLDYPGV GPEHAYLKDT GRVIYKAIND SEALDAFRLL THTEGIIPAL ESSHAVAYAI QLAKTMSKEQ SIIVNLSGRG DKDMHTVAAI D  
PCR Forward Primer
TACTTCCAATCCAATGCCGAGCTTCCAGATGAGTTTGGCCATTTT  
PCR Backward Primer
TTATCCACTTCCAATGTTAGTCAATAGCAGCTACTGTATGCATATCTTTAT  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-12 2016-05-12 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-21 2016-05-23 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production