Clone

Target
IDP95855  
Clone Suffix
1
Experiment Start Date
Feb 16, 2017  
Experiment End Date
Feb 16, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
117  
Reference Sequence Used
MDRVFIEGLE VDTVIGVYDW ERGIRQCLRL DLTLGWDNRP AAAGDDLALA LDYAALSERV QEFARESHFQ LVETFAERLA EVLMGERGIP WLRIRVTKPG AVPAARGVGV EIERGCR  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MDRVFIEGLE VDTVIGVYDW ERGIRQCLRL DLTLGWDNRP AAAGDDLALA LDYAALSERV QEFARESHFQ LVETFAERLA EVLMGERGIP WLRIRVTKPG AVPAARGVGV EIERGCR  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGACAGAGTTTTTATCGAAG  
PCR Backward Primer
TTATCCACTTCCAATGTCAGCGACATCCGCGTTCG  
Shipped To Lab
ANL  
Shipped Date
Mar 20, 2017  
Inserted
Mar 27, 2017  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-02-16 2017-02-16 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay