Clone

Target

IDP95872  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Feb 16, 2017  

Experiment End Date

Feb 16, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

355  

Reference Sequence Used

MVKLDRYIGV TVFVAILAVL GVILGLALLF AFIDELNDIS ASYGIGDALR FIFLTAPRRA YDMLPMAALI GCLVGLGTLA SNSELTIMRA AGVSLSRIVW AVMKPMLVLM LAGILVGEYV APWTENIAQS GRALAQGGGD SQSSKRGLWH RQGREYIHIN AVQPNGVLYG VTRYRFDEQR GLESASFAKR ARFETDHWQL EEVTTTLLHP REKRSEVVKL PTERWDAQLS PQLLNTVVME PEALSISGLW QYIHYLADQG LNNNRYWLAF WTKVLQPLVT AALVLMAISF IFGPLRSVTL GQRIFTGVLV GFVFRIAQDL LGPSSLVFDF PPLLAVVIPA SICALAGVWL LRRAG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTTAAGCTCGATCGTTAC  

PCR Backward Primer

TTATCCACTTCCAATGTCAGCCCGCCCGGCGTAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 27, 2017