Clone

Target

IDP96143  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

May 05, 2017  

Experiment End Date

May 05, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

1210  

Reference Sequence Used

MDKELPWLAD NAQLELKYKK GKTPLSHRRW PGEPVSVITG SLIQTLGDEL LQKAEKKKNI VWRYENFSLE WQSAITQAIN LIGEHKPSIP ARTMAALACI AQNDSQQLLD EIVQQEGLEY ATEVVIARQF IARCYESDPL VVTLQYQDED YGYGYRSETY NEFDLRLRKH LSLAEESCWQ RCADKLIAAL PGINKVRRPF IALILPEKPE IANELVGLEC PRTHFHSKEW LKVVANDPTA VRKLEHYWSQ DIFSDREASY MSHENHFGYA ACAALLREQG LAAIPRLAMY AHKEDCGSLL VQINHPQVIR TLLLVADKNK PSLQRVAKYH KNFPHATLAA LAELLALTEP PARPGYPIIE DKKLPAQQKA RDEYWRTLLQ TLMASQPQLA AEVMPWLSTQ PQSVLKSYLS APPKPVIDGT DNSNLPEILV SPPWRSKKKM TAPRLDLAPL ELTPQVYWQP GEQERLAATE PARYFSTESL AQRMEQKSGR VVLQELGFGD DVWLFLNYIL PGKLDAARNS LFVQWHYYQG RVEEILNGWN SPEAQLAEQA LRSGHIEALI NIWENDNYSH YRPEKSVWNL YLLAQLPREM ALTFWLRINE KKHLFAGEDY FLSILGLDAL PGLLLAFSHR PKETFPLILN FGATELALPV AHVWRRFAAQ RDLARQWILQ WPEHTASALI PLVFTKPSDN SEAALLALRL LYEQGHGELL QTVANRWQRT DVWSALEQLL KQGPMDIYPA RIPKAPDFWH PQMWSRPRLI TNNQTVTNDA LEIIGEMLRF TQGGRFYSGL EQLKTFCQPQ TLAAFAWDLF TAWQQAGAPA KDNWAFLALS LFGDESTARD LTTQILAWPQ EGKSARAVSG LNILTLMNND MALIQLHHIS QRAKSRPLRD NAAEFLQVVA ENRGLSQEEL ADRLVPTLGL DDPQALSFDF GPRQFTVRFD ENLNPVIFDQ QNVRQKSVPR LRADDDQLKA PEALARLKGL KKDATQVSKN LLPRLETALR TTRRWSLADF HSLFVNHPFT RLVTQRLIWG VYPANEPRCL LKAFRVAAEG EFCNAQDEPI DLPADALIGI AHPLEMTAEM RSEFAQLFAD YEIMPPFRQL SRRTVLLTPD ESTSNSLTRW EGKSATVGQL MGMRYKGWES GYEDAFVYNL GEYRLVLKFS PGFNHYNVDS KALMSFRSLR VYRDNKSVTF AELDVFDLSE ALSAPDVIFH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGACAAGGAATTACCGTGG  

PCR Backward Primer

TTATCCACTTCCAATGTTAATGGAAAATGACGTCAGGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 17, 2017