Clone

Target

IDP96297  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

120  

Sequence End

525  

Reference Sequence Used

PMRITEDGYS AWLTRPNELG FNRNMAVLGQ GHYLVLVDPA SLVDVIPFAD ISIDAALVAN ATHLVFSSSS KLDPHIRNMI KDQEMVSVEY NGAMYVMKPV PELGINVVAW AALKPLAETW HRQLLFWLPF GMLISLLAAL FVLRILRRIQ SPRNRLLDAI NNREFEVYYQ PIVALCSGKI VGAEALTRWP QPDGSSLSSD SFVPLAEQTG LISRLTHLVI EKVFEDMGNW LHLHADQHIS VNLAPADLTS GKLPPLLSQL LNKWQVRPEQ IALELTERGF ADPKISAPAI AAFRRSGHAI YIDDFGTGYS SLSYLQDLDV DTLKIDKSFV DALEYKNVTP HIIGMAKSLK LAMVAEGVET AGQLAWLHRH GVQYGQGWYY SKALPKAEFM AWAENNLNAP STSDKI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGCCCATGCGCATTACGGAAG  

PCR Backward Primer

TTATCCACTTCCAATGTCAAATCTTATCTGAAGTAGAAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017