Clone

Target

IDP96306  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

78  

Sequence End

440  

Reference Sequence Used

LSPWGNASVN LLVDDEGNFN GSSGSWFIPW NDNNRYLSWS QLGLTQQSDG LVSNAGIGQR WVAGKWLLGY NTFYDNLLDE NLQRAGLGAE AWGENLRLSA NYYQPLASWR ESSDVQEQRM ARGYDVTAKA WLPWFHHFNT SVSFEQYFGD NVDLFNSGTG YHNPVAVNLG LNYTPVPLVT LTAAHKQGES GASQNNLGLK LNYRFGVPLS KQLSASEVAA TRSLRGSRYD SPERDNLPVM EFRQRKTLSV WLATPPWDLK GGETVMLKLQ VRSTHGIRQI HWQGDTQALS LTAPANTHSS DGWSVIMPAW DDSDGAKNRW HLSAVVEDEK GQRVSSNEIT LTVVQPLVAL PDDDPRWKLL PDE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGCTCTCGCCCTGGGGCAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCATCCGGGAGTAACTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017