Clone

Target

IDP96405  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

85  

Sequence End

304  

Reference Sequence Used

DAFEAINGTH GVNLTRHNYP ALHGTLQTAA TQCTDNLDDA LLLPAFDQAV SINRSQDDHS HGLGTLELKF RYYVDLNKHY VYFYDLINSR RFAMHRWTFL QKGTMGINRK DIDKLFTGRT VISSIYMDDI TQENVMSFLT PVYLAGSLKG IVMVDVNQDN LKNIFYTQDR PLVWRYLNVT LKDMDSGKEI IINQSKNNLF QYVNYSHDIP GGLRVSLSLD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGACGCCTTTGAAGCTATTAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAATCAAGGGACAACGAAACG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017