Clone

Target
IDP96412  
Clone Suffix
1
Experiment Start Date
Jul 28, 2017  
Experiment End Date
Jul 28, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
128  
Reference Sequence Used
MYDNLKSLGI TNPEEIDRYS LRQEANNDIL KIYFQKDRGE FFAKSVKFKY PRQRKTVVAD GIGQGYKEVQ EISPNLRYVI DELDQICQRD RSELDLKRKI LDDLRHLESV VANKISEIEA DLDKLTRK  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MYDNLKSLGI TNPEEIDRYS LRQEANNDIL KIYFQKDRGE FFAKSVKFKY PRQRKTVVAD GIGQGYKEVQ EISPNLRYVI DELDQICQRD RSELDLKRKI LDDLRHLESV VANKISEIEA DLDKLTRK  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTATGACAATCTAAAAAGTCTG  
PCR Backward Primer
TTATCCACTTCCAATGTTACTTACGGGTAAGCTTGTC  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-07-28 2017-07-28 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay