Clone

Target

IDP96419  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

347  

Reference Sequence Used

MDNDRQKALD TVIKNMEKSF GKGAVMKLGD NIGRRVSTTS TGSVTLDNAL GVGGYPKGRI IEIYGPESSG KTTVALHAIA EVQSNGGVAA FIDAEHALDP EYAQALGVDI DNLYLSQPDH GEQGLEIAEA FVRSGAVDIV VVDSVAALTP KAEIEGEMGD THVGLQARLM SQALRKLSGA ISKSNTTAIF INQIREKVGV MFGNPETTPG GRALKFYSSV RLEVRRAEQL KQGQEIVGNR TKIKVVKNKV APPFRVAEVD IMYGQGISKE GELIDLGVEN DIVDKSGAWY SYNGERMGQG KENVKMYLKE NPQIKEEIDR KLREKLGISD GDVEETEDAP KSLFDEE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATAACGATCGTCAAAAAG  

PCR Backward Primer

TTATCCACTTCCAATGCTATTCTTCGTCAAATAATGACT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017