Clone

Target
IDP96159  
Clone Suffix
103
Sequence Comments
IDP96159.18-542.P(542)..pMCSG53  
Experiment Start Date
Jun 04, 2018  
Experiment End Date
Jun 04, 2018  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG53  
Sequence Start
18  
Sequence End
542  
Reference Sequence Used
ATGAGIARDC ALRGLRVILV ERHDIATGAT GRNHGLLHSG ARYAVTDAES ARECISENQI LKRIARHCVE PTNGLFITLP EDDLSFQATF IRACEEAGIS AEAIDPQQAR IIEPAVNPAL IGAVKVPDGT VDPFRLTAAN MLDAKEHGAV ILTAHEVTGL IREGATVCGV RVRNHLTGET QALHAPVVVN AAGIWGQHIA EYADLRIRMF PAKGSLLIMD HRINQHVINR CRKPSDADIL VPGDTISLIG TTSLRIDYNE IDDNRVTAEE VDILLREGEK LAPVMAKTRI LRAYSGVRPL VASDDDPSGR NVSRGIVLLD HAERDGLDGF ITITGGKLMT YRLMAEWATD AVCRKLGNTR PCTTADLALP GSQEPAEVTL RKVISLPAPL RGSAVYRHGD RTPAWLSEGR LHRSLVCECE AVTAGEVQYA VENLNVNSLL DLRRRTRVGM GTCQGELCAC RAAGLLQRFN VTTSAQSIEQ LSTFLNERWK GVQPIAWGDA LRESEFTRWV YQGLCGLEKE QKDAL  
PCR Forward Primer
TACTTCCAATCCAATGCCGTTTACACCTCAATAAGCCAACAGTGGT  
PCR Backward Primer
TTATCCACTTCCAATGTTACTCATCGTGTTGTTCCGCTTCGT  
Shipped To Lab
ANL  
Shipped Date
Jun 04, 2018  
Inserted
Jun 09, 2018  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2018-06-04 2018-06-04 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2018-06-28 2018-06-28 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay