Clone

Target

IDP50823  

Clone Suffix

105

Sequence Comments

IDP50823.6-856.P(859)..pMCSG53  

Experiment Start Date

Oct 08, 2018  

Experiment End Date

Oct 08, 2018  

Person

Michael Endres  

Lab

Argonne National Laboratory  

Protocol ID

standard cloning_ANL  

Status

success  

Vector

pMCSG53  

Sequence Start

6  

Sequence End

856  

Reference Sequence Used

LTAKFQQALA EAQSLALAAD SSYLEAGFVL KALLDDQNSG AAALLAHAGV NVPQVKQRLQ QHLNSLPKVS GQGGEILPSR ELQAVLNLMD KAATKRGDAY IASELFLLAL VQQNDAAGKI LKEAGATEQN INAAIDAVRG GQNVNDANAE DQRDALKKYT LDLTQRARDG KLDPVIGRDD EIRRAIQVLQ RRTKNNPVLI GEPGVGKTAI VEGLAQRIVN GEVPESLRNK RLLVLDLAAL IAGAKYRGEF EERLKGVLND LAKDDGNTLI FIDEIHTLVG AGKTDGAMDA GNMLKPALAR GELHCIGATT LDEYRQYIEK DAALERRFQK VLVGEPSVED TIAILRGLQE RYEIHHGIDI TDPAIVAAAE LSDRYITDRF LPDKAIDLID EAASRVKMEK ETKPEAMDKI DRRLIQLRME KAHVEKEKDD ASKKRLELID EEIDGLQKEY ADLDEIWKAE KAISDGAANI KKQIDEVKIK IEQAKRQGDL ALASKLMYED LEHLEKQRAA AERADTDSTK PANKLLRNNV GAEEIAEVVS RMTGIPVSKM MEGERDKLLK MEEVLHRRVV GQNEAVRAVS DAIRRSRSGL ADPNKPYGSF LFLGPTGVGK TELCKALAGF LFDSEDHLIR IDMSEYMEKH AVARLIGAPP GYVGYEEGGY LTEQVRRKPY SVILLDEVEK AHPDVFNILL QVLDDGRLTD GQGRTVDFKN TVIVMTSNIG SQHIQQMGTQ DYEAVKEVVM EDVKEHFRPE MINRIDEVVV FHGLDQANIR SIAKIQLKGL EKRLEKQNLR LTVSDAALDI IAKAGFDPIY GARPLKRAIQ SEIENPLAKA LLAGNYAPES EIKVEADGDR L  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCCTTAACCGCCAAATTCCAACAAGCCCTT  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGTCTGTCGCCGTCGGCTT  

Shipped To Lab

ANL  

Shipped Date

Dec 20, 2018  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2018