Clone

Target
IDP97103  
Clone Suffix
106
Sequence Comments
IDP97103.25-164.P(644)..pMCSG53  
Experiment Start Date
Feb 04, 2019  
Experiment End Date
Feb 04, 2019  
Person
Michael Endres  
Lab
Northwestern University  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG53  
Sequence Start
25  
Sequence End
164  
Reference Sequence Used
ERQTALQKFA RYNDSWAVSQ TVAEYLRLQN KLAVFALNPG KIDRDELQLR LDIMLSRLEL LKQGNLGAFI NEKQQWRDLV TRLDAILAHL EQEVNTLDPA EVPDLLAAMS ALDAPMTTLA SSSVAYDVNL IDTAHEDVRK  
PCR Forward Primer
TACTTCCAATCCAATGCCGAGCGGCAGACCGCTCTG  
PCR Backward Primer
TTATCCACTTCCAATGTTACTTGCGAACATCCTCATGGGC  
Shipped To Lab
NU  
Shipped Date
Feb 11, 2019  
Inserted
Feb 05, 2019  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2019-02-08 2019-02-08 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2019-09-25 2019-09-27 Sergii Pshenychnyi Northwestern University NU_SelMet_expression success E.coli SeMet IPTG production
2019-07-30 2019-08-01 Grant Wiersum Northwestern University NU_SelMet_expression success E. coli SeMet IPTG production