Clone

Target

IDP00444  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

671  

Reference Sequence Used

MNPFIVADAE SCIGCRSCEV ACVVAHHEGQ FPDKPEYFTP RVKVFKGDQC ATAVFCHHCE DAPCASTCPN GAIVELNNRV QVIQEKCIGC KTCMIACPFG MMTVVTETVQ PASHRLADVY RRTEAQKCDL CLDQPDGPAC IKTCPTQALT LVDQQYLLHQ QQQKRQHMAL NEHNGRLFSR AVVQAASASS NKSIRDATPV NLLQRPRAPR LEPKKIPLAE RKTGFAEIYL PFTESQIHEQ AERCLECGDK TICQWTCPLH NAIPKWISLA HQGRIHEAAE LSHQTSSLPE VCGRVCPQDR LCEQSCTLND HDGAVTIGQI ERHITETALA TGWRPDLSQV KPSGKRVAII GAGPAGLGCA DILVRNGITP VVFDRYPEIG GLLTFGIPAF KLEKSVMTRR REIFTEMGVE FCLNTEIGRD ITMENLLHDF DALFLGVGTY RSMRSGLENE DAQGVYDALP FLIGNTQHLM GYTANGAHPY VSMQDQRVVV LGGGDTAMDC VRTSLRQGAS RVVCAYRRDE KNMPGSKREV KNAREEGAEF MFNLQPMSIV VDESGQVTGI KMVRTEMGQA DAQGRRQALP ITGSEHLIPA DAVIMAFGFS PHRMSWLAEH NVVLDKHGRV VAPTISGYPY QTSNPKIFAG GDIVRGADLI VTAIAEGRKA AESIAYYLNV L  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATCCATTTATTGTTGCTGA  

PCR Backward Primer

TTATCCACTTCCAATGTTATAAAACGTTCAAATAATACGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008