Clone

Target

IDP02702  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jan 28, 2009  

Experiment End Date

Jan 29, 2009  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

565  

Reference Sequence Used

MNNFVKLGLT TGVVLSAIIP YGGVHAATED LKVETKEDTF RTGNLTAPSQ KSAENVVKDA LKGKTEQALS SKQVNTESKV NYNVTQSRKS YDGTTLVRLQ QTYEGRDVYG YQLTAHINDD GVLTSISGDS AQDLQQQEDL KQPIILSEED AKKQLFKIYG DNLTFVEEPE IKQVVYVDEN TNKATNAYQI TFSASTPEYV SGTVLIDAFV GDLLKELVQK LGIQVDSSIV QSATANKSQD PSKLTGTGKD DLGMNRTFGI TQRSDGTYTL ADYSRGKGIE TYTANYKDYN NYRRNVWGYL DDLVTSNSTN FTDPKAVSAH YLATKVYDFY KEKYSRNSFD NNGQKVISVV HGWNTNGKNI GNPKQWFNAF SNGAMLVYGD PIVRAFDVAG HEFTHAVTRN ESGLEYAGEA GAINEALSDI LGVAVEKYAN NGKFNWTMGE QSGRIFRDMK NPSSISSRYP EDYRHYNNLP IDADHDYGGV HTNSSIINKV AYLIASGGNH NGVNVQGIGE DKMFDIFYYA NTDELNMTSD FKELKEACIR VAMNLYGKDS LEVQAVQQAF KAAYI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATAATTTCGTAAAGTTAGGT  

PCR Backward Primer

TTATCCACTTCCAATGTTAAATATAAGCTGCTTTAAATGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 24, 2009