Clone

Target

IDP00122  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

69: L -> S  

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

380  

Reference Sequence Used

MKIVIAPDSY KESLSALEVA TAIEQGFREI WPDADYLKLP LADGGEGTVE AMVEATAGRI VHVEVTGPLG HRVNAFYGLS GDARSAFIEM AAASGLEQVP PAQRDPLKTT SWGTGELIRH ALDAGVEHII IGIGGSATND GGAGMVQALG ARLRDAQGND IAQGGIGLET LASIDISGLD KRLSACHIEV ACDVTNPLTG KEGASAVFGP QKGATPEMIE RLDTALTRYA HLIARDLHVD VLDLAGGGAA GGMGAALYAF CGAQLRRGIE IVTDALHLEA CLADADLVIT GEGRIDSQTI HGKVPIGVAN IAKRYNKPVI GIAGSLTADV SVVHEHGLDA VFSVIYTICT LEDALKNASE NVRMTARNVA ATLKAGQQLR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKIVIAPDSY KESLSALEVA TAIEQGFREI WPDADYLKLP LADGGEGTVE AMVEATAGRI VHVEVTGPSG HRVNAFYGLS GDARSAFIEM AAASGLEQVP PAQRDPLKTT SWGTGELIRH ALDAGVEHII IGIGGSATND GGAGMVQALG ARLRDAQGND IAQGGIGLET LASIDISGLD KRLSACHIEV ACDVTNPLTG KEGASAVFGP QKGATPEMIE RLDTALTRYA HLIARDLHVD VLDLAGGGAA GGMGAALYAF CGAQLRRGIE IVTDALHLEA CLADADLVIT GEGRIDSQTI HGKVPIGVAN IAKRYNKPVI GIAGSLTADV SVVHEHGLDA VFSVIYTICT LEDALKNASE NVRMTARNVA ATLKAGQQLR  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATAGTGATCGCACCAG  

PCR Backward Primer

TTATCCACTTCCAATGTCAGCGTAACTGTTGCCCC  

Shipped To Lab

ANL  

Shipped Date

May 04, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009