Clone

Target

IDP00356  

Clone Suffix

3 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

457  

Reference Sequence Used

MSKRYAVVLA AGQGTRMKSK LYKVLHPVCG KPMVEHVVDQ ISTLNVDKVV TIVGHGAEKV QEHLAGKSEF VKQDEQLGTA HAVLQAKAEL AGKDGVTLVV CGDTPLIEAS TMEALLKYHH EKRAKATILT TVIEDPTGYG RIIRDDLGIV EKIVEHKDAT EKEQRISEIN TGTYCFDNKA LFEALENVSN DNVQGEYYLP DVIKILKDSD EVVAAYRMES FEESLGVNDR IALAEASKLM QRRINENHMR NGVTLVNPES TYIDIDVKIG QDTVIEPGVM LRGKTVIGDD CVVTSGSEIV NSVIGERVHV RTSSIFESKV GDDVQIGPYA HLRPESDIHD HVKIGNYVET KKAVVGEGTK LPHFIYMGDA EIGKNVNVGC GSIAVNYDGK NKAKTIIGDN VFVGCNSNLI APVKVGDRAF IAAGSTITKD VPEDALGIAR AKQDNKLGYA KHLNHSK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSKRYAVVLA AGQGTRMKSK LYKVLHPVCG KPMVEHVVDQ ISTLNVDKVV TIVGHGAEKV QEHLAGKSEF VKQDEQLGTA HAVLQAKAEL AGKDGVTLVV CGDTPLIEAS TMEALLKYHH EKRAKATILT TVIEDPTGYG RIIRDDLGIV EKIVEHKDAT EKEQRISEIN TGTYCFDNKA LFEALENVSN DNVQGEYYLP DVIKILKDSD EVVAAYRMES FEESLGVNDR IALAEASKLM QRRINENHMR NGVTLVNPES TYIDIDVKIG QDTVIEPGVM LRGKTVIGDD CVVTSGSEIV NSVIGERVHV RTSSIFESKV GDDVQIGPYA HLRPESDIHD HVKIGNYVET KKAVVGEGTK LPHFIYMGDA EIGKNVNVGC GSIAVNYDGK NKAKTIIGDN VFVGCNSNLI APVKVGDRAF IAAGSTITKD VPEDALGIAR AKQDNKLGYA KHLNHSK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCAAAACGATATGCTGTAG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTACTATGATTCAAATGTTTC  

Shipped To Lab

NU  

Shipped Date

May 04, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009