Clone

Target

IDP00316  

Clone Suffix

1

Sequence Comments

273: I -> V  

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

463  

Reference Sequence Used

MLTPTRAFYQ LLDLYPTAIL LEFSPRNEKP YAFIGFNPLA QIIARGDEIT VFLKNTVTKM KGDPFHHLRR LRQQINCIDN HPLTKLTGAA IGYLSYDAIR YVESIPDRHT ASEKIADIDF KFYRDGITFD LESNNAIVSH IVEVKDELKH CYEYGLEKLA DITNNLFTPA RPKLSTNRKT HYSKSTSLDF KPDSNDTDFK KIVNKAKQYI AAGDIFQVVS SRTFKKPYQG KPFDIYRALK KVSPSPYHFY LQNDSLAIAG ASPEKMVSVK NGIIDSVPLA GTRPRGNGKS DQTLAQELIN DPKETAEHLM LVDLARNDIG IVSQAGSVEV AELMQPVNFS HVMHLASRVQ VSLAKNYDAI DVLKATFPAG TLSGAPKIRA MEIIDELEHS RRGLYGGAIV ALDRDDNLDT CIIIRTTIVE NGIASIRAGA GIVHDSDPIA EANETRHKAK SVMAAIQLTE GGL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLTPTRAFYQ LLDLYPTAIL LEFSPRNEKP YAFIGFNPLA QIIARGDEIT VFLKNTVTKM KGDPFHHLRR LRQQINCIDN HPLTKLTGAA IGYLSYDAIR YVESIPDRHT ASEKIADIDF KFYRDGITFD LESNNAIVSH IVEVKDELKH CYEYGLEKLA DITNNLFTPA RPKLSTNRKT HYSKSTSLDF KPDSNDTDFK KIVNKAKQYI AAGDIFQVVS SRTFKKPYQG KPFDIYRALK KVSPSPYHFY LQNDSLAIAG ASPEKMVSVK NGVIDSVPLA GTRPRGNGKS DQTLAQELIN DPKETAEHLM LVDLARNDIG IVSQAGSVEV AELMQPVNFS HVMHLASRVQ VSLAKNYDAI DVLKATFPAG TLSGAPKIRA MEIIDELEHS RRGLYGGAIV ALDRDDNLDT CIIIRTTIVE NGIASIRAGA GIVHDSDPIA EANETRHKAK SVMAAIQLTE GGL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTTACGCCAACCCGGG  

PCR Backward Primer

TTATCCACTTCCAATGTTATAATCCTCCTTCGGTCAG  

Shipped To Lab

UT  

Shipped Date

Jul 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009