Clone

Target
IDP01352  
Clone Suffix
2
Experiment Start Date
Jun 06, 2008  
Experiment End Date
Jun 07, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG19c  
Sequence Start
1  
Sequence End
230  
Reference Sequence Used
MLGYAFPSIL GNLMSKIYQM DAVDWLKTLE NCSVDLFITD PPYESLEKYR QIGTTTRLKE SKSSSNQWFS VFPNTRFEEL FREVYRVLKK GSHFYLFCDQ ETMFLAKPIA ESVGFKFWKP IVWDKCAIGM GYHYRARYEF ILFFEKGKRK LNDLSVPDVL EYKRVWKGYP TEKPVELLEV LIRQSSSENE IVADSFFGSG STLIAANNLS RKYIGCDISS SAHEYFKNRA  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTTAGGTTATGCGTTTCCC  
PCR Backward Primer
TTATCCACTTCCAATGTCAAGCTCTATTCTTAAAATACTC  
Shipped To Lab
ANL  
Shipped Date
Jul 16, 2008  
Inserted
Aug 08, 2008  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-06-16 2008-06-17 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay
2008-04-09 2008-04-11 Natalia Maltseva Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 0.5 mM IPTG production
2008-10-07 2008-10-07 Natalia Maltseva Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 0.5 mM IPTG production