Clone

Target

IDP04700  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

215  

Reference Sequence Used

MKAYQREFIE FALNKQVLKF GEFTLKSGRI SPYFFNAGLF NTGLDLAKLG RFYAAALMDC GVEFDLLFGP AYKGIPIATT TAVALAEHHE RDVPYCFNRK EAKTHGEGGN LVGSPLQGRV MLVDDVITAG TAIRESMEII NAQGATLAGV MISLDRQERG RGEISAIQEV ERDYHCKVIA IVTLNDVIRY LEDKPEMAEH LVAVRQYREQ YGVTL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGCCTATCAGCGCGA  

PCR Backward Primer

TTATCCACTTCCAATGCTAAAGGGTAACCCCATATTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009